Work

Stimulation of human eosinophil and neutrophil polymorphonuclear leukocyte chemotaxis and random migration by 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid.

Year: 1977

Type: article

Abstract: A C T A htuman platelet lipoxygenase-gen- erated prodtuct of arachidonic acid, identified by thin- layer chromiiatographic and mass spectrometric proper- ties as 12-L-hydroxy-5,8, 10,14-eicosatetraenoic acid (HETE), wvas selectively chemotactic in vitro for human polymnorplhontuclear leuikocytes (PMN), as compared to moniontuclear leukocytes, with a preference for eosinophils.Preincubation of PMN with partially- purified HETE at peak chemotactic concentrations of 8-24 /,g/ml redtuced their random and chemotactic migration and stimulated the activity of their hexose monophosphate shunt; minimally chemotactic concen- trations of 0.03-1 ,ug/ml enhanced PMN random migration without influiencing other functions.HETE may thtus be capable of preferentially attracting eosino- phils to foci of tisstue reaction associated with platelet activation.'Abbreviationis tised in this paper: HBSS-ovalbumin, Hanks' balanced salt solution made 0.4% in ovalbumin; HETE, 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid; HMPS, hexose monophosphate shunt; hpf, high power field; PMN, polymorphonuclear leukocyte(s).

Source: Journal of Clinical Investigation

DOI: 10.1172/jci108617

Authors: E J Goetzl,, J M Woods, R R Gorman

Institution: Harvard University

Language: English

Topic: Eicosanoids and Hypertension Pharmacology

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: National Institutes of Health