Work

Simultaneous Collision Induced Dissociation of the Charge Reduced Parent Ion during Electron Capture Dissociation

Year: 2009

Type: article

Abstract: A method of performing collision induced dissociation (CID) on the charge-reduced parent ion as it is formed during electron capture dissociation (ECD), called ECD+CID, is described. In ECD+CID, the charge-reduced parent ion is selectively activated using resonant excitation and collisions with the helium bath gas inside a linear quadrupole ion trap ECD device (ECD(LIT)). It has been observed that ECD+CID can improve the sequence coverage for beta-endorphin over performing ECD alone (i.e., from 72 to 97%). Perhaps just as important, ECD+CID can be used to reduce the extent of multiple electron capture events observed when performing ECD in the ECD(LIT). Consequently, the abundance of mass-to-charge ratios corresponding to ECD product ions that contain neutralized protons is decreased, simplifying the interpretation of the product ion spectrum.

Source: Analytical Chemistry

DOI: 10.1021/ac900627n

Authors: Jared M. Bushey, Takashi Baba, Gary L. Glish

Institutions: University of North Carolina at Chapel Hill, Hitachi (Japan)

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Center for Research Resources

Awards: G158619212, G2188690378, Instrument for Determining Intramolecular Interactions