Work

Identification and Analysis of a Functional Human Homolog of the SPT4 Gene of Saccharomyces cerevisiae

Year: 1996

Type: article

Abstract: Spt4p is a nonhistone protein of Saccharomyces cerevisiae that is believed to be required for normal chromatin structure and transcription. In this work we describe the isolation and analysis of a human gene, SUPT4H, that encodes a predicted protein 42% identical to Spt4p. When expressed in S. cerevisiae, SUPT4H complemented all spt4 mutant phenotypes. In human cells SUPT4H encodes a nuclear protein that is expressed in all tissues tested. In addition, hybridization analyses suggest that an SUPT4H-related gene is also present in mice. SUPT4H was localized to human chromosome 17 by PCR analysis of a human-rodent somatic cell hybrid panel. Thus, like other proteins that are components of or control the structure of chromatin, Spt4p appears to be conserved from S. cerevisiae to mammals.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.16.6.2848

Authors: Grant A. Hartzog, Munira A. Basrai, Stephanie L. Ricupero-Hovasse, Philip Hieter, Fred Winston

Institutions: Harvard University, Johns Hopkins University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: G1890337441, Program on Molecular and Cellular Mechanisms of Tumorigenesis, G5921122885, Molecular Genetics of Tumor Viruses and Cells