Work

Tobacco mosaic virus rods and spheres as supramolecular high-relaxivity MRI contrast agents

Year: 2013

Type: article

Abstract: To compensate for the low sensitivity of magnetic resonance imaging (MRI), nanoparticles have been developed to deliver high payloads of contrast agents to sites of disease. Here, we report the development of supramolecular MRI contrast agents using the plant viral nanoparticle tobacco mosaic virus (TMV). Rod-shaped TMV nanoparticles measuring 300 × 18 nm were loaded with up to 3500 or 2000 chelated paramagnetic gadolinium(III) ions selectively at the interior (iGd-TMV) or exterior (eGd-TMV) surface, respectively. Spatial control is achieved through targeting either tyrosine or carboxylic acid side chains on the solvent exposed exterior or interior TMV surface. The ionic T1 relaxivity per Gd ion (at 60 MHz) increases from 4.9 mM−1 s−1 for free Gd(DOTA) to 18.4 mM−1 s−1 for eGd-TMV and 10.7 mM−1 s−1 for iGd-TMV. This equates to T1 values of ∼30 000 mM−1 s−1 and ∼35 000 mM−1 s−1 per eGd-TMV and iGd-TMV nanoparticle. Further, we show that interior-labeled TMV rods can undergo thermal transition to form 170 nm-sized spherical nanoparticles containing ∼25 000 Gd chelates and a per particle relaxivity of almost 400 000 mM−1 s−1 (15.2 mM−1 s−1 per Gd). This work lays the foundation for the use of TMV as a contrast agent for MRI.

Source: Journal of Materials Chemistry B

DOI: 10.1039/c3tb00461a

Authors: Michael A. Bruckman, Stephen Hern, Kai Jiang, Chris A. Flask, Xin Yu, Nicole F. Steinmetz

Institution: Case Western Reserve University

Language: English

Topic: Lanthanide and Transition Metal Complexes

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Biomedical Imaging and Bioengineering