Work

Fatty acid acylated Fab‐fragments of antibodies to neurospecific proteins as carriers for neuroleptic targeted delivery in brain

Year: 1991

Type: article

Abstract: A method for targeted delivery of neuroleptics from blood in brain based on using Fab-fragments of antibodies to antigens of brain glia cells (acid gliofibrillar antigen and alpha 2-glycoprotein) is suggested. The essence of the technique is that the molecule of neuroleptic (trifluoperazine) is conjugated with Fab-fragments of these antibodies. The conjugate thus obtained is modified by stearoylchloride in the system of Aerosol OT reversed micelles in octane. The study of the distribution of 125I-labelled conjugates in the rat organism after intracordial introduction is performed. On the contrary to the nonmodified conjugates and conjugate, containing fatty acylated Fab-fragments of antibodies, nonspecific to the rat brain, the conjugate of trifluoperazine with stearoylated Fab-fragments of antibodies to neurospecific antigens accumulate in brain tissues. The drastic increase of the neuroleptic activity of trifluoperazine resulting from its coupling with stearoylated Fab-fragments of antiglial antibodies is observed.

Source: FEBS Letters

DOI: 10.1016/0014-5793(91)80037-4

Authors: Vladimir P. Chekhonin, Alexander V. Kabanov, Yurii A. Zhirkov, Georgii V. Morozov

Institution: Russian Research Center for Molecular Diagnostics and Therapy

Language: English

Topic: Barrier Structure and Function Studies

Subfield: Neurology

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2070187725

FWCI: 0.776

Cites: 14

Cited by: 48

Related to: 10