Work

10. Adeno-Associated Virus Type-1 Vectors Transduce Human Polarized Airway Epithelia without Polarity Preference

Year: 2006

Type: article

Abstract: The choice of appropriate adeno-associated virus serotypes for gene therapy to the human lung can differ depending on the animal model and model system in which such serotypes are evaluated. In the present study, we sought to compare the efficiency of rAAV2/1, rAAV2/2, and rAAV2/5 vector transduction in polarized human airway epithelia. Since similar vector purity is paramount to such a comparison, we developed a uniform procedure for purification of these serotype vectors using an adenovirus-free packaging system and HPLC.

Source: Molecular Therapy

DOI: 10.1016/j.ymthe.2006.08.020