Work

High‐throughput generation of tagged stable cell lines for proteomic analysis

Year: 2009

Type: article

Abstract: We present an optimized system for rapid generation of localization and affinity purification-tagged mammalian stable cell lines that facilitates complex purification and interacting protein identification. The improved components of this method, including the flexibility of inducible expression, circumvent issues associated with toxicity, clonal selection, sample yields and time to data acquisition. We have applied this method to the study of cell-cycle regulators and novel microtubule-associated proteins.

Source: PROTEOMICS

DOI: 10.1002/pmic.200800873

Authors: Jorge Z. Torres, Julie J. Miller, Peter K. Jackson

Institutions: Genentech, Stanford Medicine, Stanford University

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Cyclin Control DNA Replication and Mitosis, G1415787600, G6169187270, G8286170774