Work

Detection and Relative Quantification of Proteins by Surface Enhanced Raman Using Isotopic Labels

Year: 2008

Type: article

Abstract: Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix format without interference from protein features.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja800772p

Authors: Shirshendu K. Deb, Brandon Davis, Giselle M. Knudsen, Ravindra Gudihal, Dor Ben-Amotz, V. Jo Davisson

Institution: Purdue University West Lafayette

Language: English

Topic: Gold and Silver Nanoparticles Synthesis and Applications

Subfield: Electronic, Optical and Magnetic Materials

Field: Materials Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,500