Work

Collagenase as an agent for dissolving the zona pellucida of hamster and mouse oocytes

Year: 1991

Type: article

Abstract: Summary. Crude preparations of collagenase, which have been used commonly for tissue dissociation, contain proteases that dissolve zonae pellucidae of hamster and mouse oocytes without reducing the ability of the oolemma to fuse with spermatozoa. This gentle proteolytic removal of zona is particularly useful for the study of sperm–oocyte fusion in mice, as trypsin, chymotrypsin and pronase damage the mouse oolemma. Keywords: collagenase; zona pellucida; oocyte; spermatozoa; fusion; mouse; hamster

Source: Reproduction

DOI: 10.1530/jrf.0.0930515

Authors: M. Zuccotti, U. A. Urch, R. Yanagimachi

Institutions: University of Hawaiʻi at Mānoa, University of Hawaii System, University of California, Davis

Language: English

Topic: Reproductive Biology and Fertility

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

Funders: National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: G5192142844, ANALYSIS OF FERTILIZATION MECHANISMS IN MAMMALS