Work

Amphioxus homologs of Go‐coupled rhodopsin and peropsin having 11‐cis‐ and all‐trans‐retinals as their chromophores

Year: 2002

Type: article

Abstract: Because of low contents in the native organs and failure of the expression in cultured cells, the chromophore configurations of the pigments in Go-coupled opsin and peropsin groups in the opsin family are unknown. Here we have succeeded in expression of the amphioxus homologs of these groups in HEK293s cells and found that they can be regenerated with 11-cis- and all-trans-retinals, respectively. Light isomerized the chromophores of these opsins into the all-trans and 11-cis forms, respectively. The results strongly suggest that the physiological function of peropsin would be a retinal photoisomerase, while 11-cis configuration is necessary for the Go-coupled opsin groups.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(02)03616-5

Authors: Mitsumasa Koyanagi, Akihisa Terakita, Kaoru Kubokawa, Yoshinori Shichida

Institutions: Kyoto University, Japan Science and Technology Agency, The University of Tokyo

Language: English

Topic: Photoreceptor and optogenetics research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2071317669

FWCI: 1.637

Cites: 34

Cited by: 115

Related to: 10