Year: 2006
Type: conference-paper
Abstract: A highly stable amylase has been identified from a hyper-thermophilic archaeon Pyrocoocus furiosus, which is suitable for the construction of Time Temperature Integrators (TTIs) for use in the food industry.Previously, amylases from terrestrial Bacilli have provided the feed stock for enzymatic TTIs.However, their application has been limited to pasteurisation; the new amylase now makes possible the application of such technology to sterilisation processes.The amylase demonstrates excellent thermal properties for its application as a "safety" TTI; namely an isothermal decimal reduction time at 121.1 o C of 22.5 ±4 minutes (21.5 ±2 under non isothermal) and a z-value of 10 ±1.5 o C.The enzymatic TTI have a distinct number of advantages over other temperature measurement technologies, e.g.thermocouples or data loggers, for the examination of continuous processes.Their small and near neutrally buoyant construction, 2.5mm x 8.0mm with 25µl of enzyme solution, makes them ideal for introduction into fluids or within food particulates.They are robust enough to pass through nearly all processing equipment; the encapsulation is from silicon tubing with elastomer plugs which also has thermal properties close to that of water.Post processing analysis is quick, inexpensive and accurate also the enzyme, an amylase is generally regarded as safe.The sterilisation TTIs have been tested in reel & spiral and Largarde steam air retorts in cans and doypacks and showed F 0 -values appropriate to the log reductions of Clostridium botulinum spores.12 Additionally, manufacturers must prove that their products and processes are
Authors: P. Cox, G. Tucker, H. Brown, S. Bakalis, P. Fryer, M. Adams
Institutions: University of Birmingham, University of Georgia
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2071396249