Work

3,4-methylenedioxymethamphetamine-induced release of serotonin and inhibition of dorsal raphe cell firing: potentiation by L-tryptophan

Year: 1990

Type: article

Abstract: The effects of the serotonin (5-HT) precursor L-tryptophan on MDMA (3,4-methylenedioxymethamphetamine)-induced inhibition of dorsal raphe neuronal firing were characterized using extracellular single-unit recording and microdialysis techniques in the in vitro midbrain slice preparation. Pretreatment with L-tryptophan (100 microM) lowered the doses of MDMA required to inhibit unit activity. Based upon IC50 values, L-tryptophan increased the potency of MDMA by approximately 3-fold. In a parallel series of experiments, microdialysis probes resting on the brain slice surface provided a means to estimate 5-HT release from the dorsal raphe nucleus. Pretreatment with L-tryptophan increased MDMA-induced 5-HT release in a manner consistent with the suppression of dorsal raphe cell firing: compared to untreated preparations, peak 5-HT release, total release and the duration of release were all increased. Taken together, these data suggest that the enhancement by L-tryptophan of MDMA-induced 5-HT release and inhibition of dorsal raphe neuronal firing is due to an increase in the amount of 5-HT available for release. The question is raised as to what effect L-tryptophan may have on the psychotropic and neurotoxic actions of MDMA.

Source: European Journal of Pharmacology

DOI: 10.1016/0014-2999(90)90110-r

Authors: Jeffrey S. Sprouse, Charles W. Bradberry, Robert H. Roth, George K. Aghajanian

Institution: Yale University

Language: English

Topic: Psychedelics and Drug Studies

Subfield: Clinical Psychology

Field: Psychology

Domain: Social Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,230