Work

A new PCR based method for the generation of nested deletions

Year: 1993

Type: article

Abstract: We have developed a simple, PCR-based protocol, random primed/anchored-PCR (RPA-PCR), that allows the selective amplification and efficient cloning of segments that are adjacent to any known sequence. We demonstrate that RPA-PCR can be used to prepare a nested set of evenly spaced deletions suitable for DNA sequencing. However, it should also be possible to use this technique for a number of other purposes: generating deletions for the analysis of eukaryotic promoters, extending cDNA clones in the 5' direction, cloning the insertion sites of retroviral proviruses and transposons, and analyzing intron/exon boundaries.

Source: Nucleic Acids Research

DOI: 10.1093/nar/21.17.4143

Authors: Jeannette M. Whitcomb, Ayoub Rashtchian, Stephen H. Hughes

Institutions: National Cancer Institute, Frederick National Laboratory for Cancer Research

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: U.S. Department of Health and Human Services, National Institutes of Health, National Cancer Institute

Awards: BASIC RESEARCH--CANCER INSTITUTE FREDERICK, G225764842