Work

Human DNA topoisomerase I-mediated cleavage and recombination of duck hepatitis B virus DNA in vitro

Year: 1999

Type: article

Abstract: In this study, we report that eukaryotic topoisomerase I (top1) can linearize the open circular DNA of duck hepatitis B virus (DHBV). Using synthetic oligonucleotides mimicking the three-strand flap DR1 region of the DHBV genome, we found that top1 cleaves the DNA plus strand in a suicidal manner, which mimics the linearization of the virion DNA. We also report that top1 can cleave the DNA minus strand at specific sites and can linearize the minus strand via a non-homologous recombination reaction. These results are consistent with the possibility that top1 can act as a DNA endo-nuclease and strand transferase and play a role in the circularization, linearization and possibly integration of viral replication intermediates.

Source: Nucleic Acids Research

DOI: 10.1093/nar/27.8.1919

Authors: P. Pourquier, Y. Pommier, A. D. Jensen, S. S. Gong, C. E. Rogler

Institutions: National Institutes of Health, National Cancer Institute, Albert Einstein College of Medicine

Language: English

Topic: Hepatitis B Virus Studies

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institutes of Health, U.S. Public Health Service, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases