Work

Induction of antigen-specific regulatory T cells in wild-type mice: Visualization and targets of suppression

Year: 2008

Type: article

Abstract: Antigen-specific transplantation tolerance in the absence of immunosuppressive drugs is a rarely achieved goal. Immune responses to Y chromosome-encoded transplantation antigens (HY) can have life-threatening consequences in the clinic. Here, we have adopted a procedure developed in T cell antigen receptor (TCR)-transgenic mice to convert naïve T cells into male-specific Foxp3(+) regulatory T cells (Tregs) in WT female mice. For this purpose, female mice were infused by osmotic minipumps with a single class II MHC-presented HY peptide and Tregs visualized by tetramer staining. As a result, animals developed Treg-mediated long-term tolerance to all HY transplantation antigens, irrespective of whether they were recognized by CD4 or CD8 T cells, on skin or hematopoietic grafts from male donors.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.0800149105

Authors: Panayotis Verginis, Katherine A. McLaughlin, Kai W. Wucherpfennig, Harald von Boehmer, Irina Apostolou

Institutions: Dana-Farber Cancer Institute, Harvard University

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: U.S. Public Health Service, National Institute of Allergy and Infectious Diseases