UM‐SCC‐104: A New human papillomavirus‐16–positive cancer stem cell–containing head and neck squamous cell carcinoma cell line
Year: 2011
Type: article
Abstract: BACKGROUND: Few human papillomavirus (HPV)(+) head and neck squamous cell carcinoma (HNSCC) cell lines exist. We established University of Michigan-squamous cell carcinoma-104 (UM-SCC-104), a new HPV(+) HNSCC cell line from a recurrent oral cavity tumor, and characterized it for the presence of cancer stem cells (CSCs). METHODS: Tumor cells were tested for biomarker expression by immunohistology, and the presence of HPV was assessed by several methods. RESULTS: UM-SCC-104 has a unique genotype, contains HPV-16, and expresses E6/E7. Inoculation of aldehyde dehydrogenase (ALDH)(+) and ALDH(-) cells in an immunocompromised mouse resulted in tumor growth from the ALDH(+) cells after 6 weeks that recapitulated the histology of the primary, whereas ALDH(-) cells did not produce tumors. CONCLUSION: UM-SCC-104, a new HPV-16, CSC-containing HNSCC cell line will aid in studying recurrent HPV(+) tumors. The aggressive nature of this tumor is consistent with high uniform expression of epidermal growth factor receptor (EGFR) and a functionally significant proportion of ALDH(+) CSCs.
Source: Head & Neck
DOI: 10.1002/hed.21962
Language: English
Topic: Head and Neck Cancer Studies
Subfield: Otorhinolaryngology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G1198238485, G1266416359, G1457163447, G2165934530, G248408221, The Molecular Basis of Head and Neck Cancer Biology, Treatment and Prevention, Advanced Research Training in Otolaryngology, G2936284972, G3405272401, Biomarkers to Guide Treatment and Improve Survival in Oral/Oropharyngeal Cancer, G3711721818, G646528168, G7158048039, Hearing, Balance and Chemical Senses Research Core Center, University of Michigan Rogel Cancer Center Support Grant 2023-2028
OpenAlex ID: W2072667482