Work

Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography

Year: 2014

Type: article

Abstract: Advances in electron cryo-tomography open up a new avenue to visualize the 3-D internal structure of a single bacterium before and after its infection by bacteriophages in its native environment, without using chemical fixatives, fluorescent dyes or negative stains. Such direct observation reveals the presence of assembly intermediates of the bacteriophage and thus allows us to map out the maturation pathway of the bacteriophage inside its host.

Source: Microbial Cell

DOI: 10.15698/mic2014.01.125

Authors: Wei Dai, Michael Schmid, Jonathan King, Wah Chiu

Institutions: Baylor College of Medicine, Massachusetts Institute of Technology

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,283

Funders: National Institutes of Health, National Eye Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases