Year: 2013
Type: article
Abstract: In recent years, the number of published genome sequences has increased substantially owing to major developments in next-generation sequencing (NGS) technologies, concomitant reduction of sequencing costs and improvements in assembly strategies. In 2011, your journal published the genome of Chinese hamster ovary (CHO)-K1 cells, the most frequently used mammalian production cell line for biopharmaceutical products 1 . In this issue, the genomes of several related CHO cell lines as well as of the genome of the Chinese hamster are also presented 2 . Although this information provides long-awaited and necessary insights for scientists working with these important production hosts, it also highlights a major drawback of short-read NGS technology, namely, the difficulty of assembling short-read data and scaffolding these sequences into a fully structured genome. This is especially critical for CHO cells, which are known to be genomically unstable, with frequent chromosome rearrangements and loss 3 , 4 . In the following correspondence, we describe how a chromosome sorting approach can facilitate genome assembly from short-read sequences.
Source: Nature Biotechnology
DOI: 10.1038/nbt.2645
Authors: Karina Brinkrolf, Oliver Rupp, Holger Laux, Florian Kollin, Wolfgang Ernst, Burkhard Linke, Rudolf Kofler, Sandrine Romand, Friedemann Hesse, Wolfgang E Budach, Sybille Galosy, Dethardt Müller, Thomas Noll, Johannes Wienberg, Thomas Jostock, Mark Leonard, Johannes Grillari, Andreas Tauch, Alexander Goesmann, Bernhard Helk, John E Mott, Alfred Pühler, Nicole Borth
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $12,690
OpenAlex ID: W2072880634