Year: 1983
Type: article
Abstract: Heteroduplex DNA molecules which contain both the wild-type and mutant sequences of a deletion nonhomology possess a characteristic electrophoretic mobility in agarose and can be readily separated from both the wild-type and deletion-containing parental homoduplex fragments. Because of the partial single stranded character of these deletion-containing heteroduplex molecules, they are selectively bound to nitrocellulose filters, and once bound, can be selectively detected by hybridization with radioactively labeled single-stranded DNA which is homologous to the sequences absent in the deletion mutation.
Source: Nucleic Acids Research
Authors: Michael J. Lichten, Maurice S. Fox
Institution: Massachusetts Institute of Technology
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Funder: Johnson and Johnson Foundation
OpenAlex ID: W2072961449