Work

In situ 3D magnetic resonance metabolic imaging of microwave‐irradiated rodent brain: a new tool for metabolomics research

Year: 2009

Type: article

Abstract: The rapid elevation in rat brain temperature achieveable with focused beam microwave irradiation (FBMI) leads to a permanent inactivation of enzymes, thereby minimizing enzyme-dependent post-mortem metabolic changes. An additional characteristic of FBMI is that the NMR properties of the tissue are close to those of the in vivo condition and remain so for at least 12 h. These features create an opportunity to develop magnetic resonance spectroscopy and imaging on microwave-irradiated samples into a technique with a resolution, coverage and sensitivity superior to any experiment performed directly in vivo. Furthermore, when combined with pre-FBMI infusion of (13)C-labeled substrates, like [1-(13)C]-glucose, the technique can generate maps of metabolic fluxes, like the tricarboxylic acid and glutamate-glutamine neurotransmitter cycle fluxes at an unprecedented spatial resolution.

Source: Journal of Neurochemistry

DOI: 10.1111/j.1471-4159.2009.05967.x

Authors: Robin A. De Graaf, Golam M. I. Chowdhury, Peter B. Brown, Douglas L. Rothman, Kevin L. Behar

Institution: Yale University

Language: English

Topic: Advanced MRI Techniques and Applications

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases