Work

Chrysanthemum stunt viroid: primary sequence and secondary structure

Year: 1981
Type: article
Abstract: The sequence of the 356 nucleotide residues of chrysanthemum stunt viroid (CSV) has been determined. Overlapping linear viroid fragments were obtained by partial ribonuclease digestion, radiolabelled in vitro at their 5'-ends, and sequenced using partial enzymic cleavage methods. Of the CSV sequence, 69% is contained in the published sequence of potato spindle tuber viroid (PSTV). Differences in the primary sequence of CSV and PSTV suggest that neither the positive nor putative negative strands of these two viroids code for functional polypeptide products. However, the two viroids can form similar secondary structures, implicating a role for viroid structure in replication.
Authors: James Haseloff, Robert H. Symons
Language: English
Subfield: Plant Science
Open Access status: green
OpenAlex ID: W2073519994
FWCI: 23.58
Cites: 17
Cited by: 165
Related to: 8