Work

A recombinant human fibronectin fragment facilitates retroviral mediated gene transfer into human hematopoietic progenitor cells

Year: 1997

Type: article

Abstract: Gene transfer into hematopoietic progenitor cells is increased when these cells are adherent to the carboxy-terminal chymotryptic fragment of human plasma fibronectin (FN30/35) containing the heparin binding domain (HBD) and the alternatively spliced type three connecting segment (IIICS) region. We report herein the production of a recombinant human fibronectin fragment comprised of the HBD and IIICS regions expressed by Sf9 insect cells following recombinant baculovirus infection. The resulting isolated peptide, HuBacFN, facilitated gene transfer into human hematopoietic cell lines and primary human hematopoietic progenitors to a level achieved with the purified human plasma FN30/35 peptide.

Source: IUBMB Life

DOI: 10.1080/15216549700203351

Authors: Parmesh Dutt, Helmut Hanenberg, Terry Vik, David A. Williams, Mervin C. Yoder

Institutions: Indiana University Bloomington, Indiana University Indianapolis, Indiana University School of Medicine, Indiana University – Purdue University Indianapolis, Howard Hughes Medical Institute

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, School of Medicine, Indiana University, National Heart, Lung, and Blood Institute, National Institute of Diabetes and Digestive and Kidney Diseases