Work

Method to reconstruct neuronal action potential train from two-photon calcium imaging

Year: 2010

Type: article

Abstract: Identification of a small population of neuronal action potentials (APs) firing is considered essential to discover the operating principles of neuronal circuits. A promising method is to indirectly monitor the AP discharges in neurons from the recordings their intracellular calcium fluorescence transients. However, it is hard to reveal the nonlinear relationship between neuronal calcium fluorescence transients and the corresponding AP burst discharging. We propose a method to reconstruct the neuronal AP train from calcium fluorescence diversifications based on a multiscale filter and a convolution operation. Results of experimental data processing show that the false-positive rate and the event detection rate are about 10 and 90%, respectively. Meanwhile, the APs firing at a high frequency up to 40 Hz can also be successfully identified. From the results, it can be concluded that the method has strong power to reconstruct a neuronal AP train from a burst firing.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.3505021

Authors: Tingwei Quan, Xiuli Liu, Xiaohua Lv, Wei R. Chen, Shaoqun Zeng

Institutions: Huazhong University of Science and Technology, Wuhan National Laboratory for Optoelectronics, The University of Texas Health Science Center at Houston

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Natural Science Foundation of China, National Institutes of Health, National Institutes of Natural Sciences, National Institute on Deafness and Other Communication Disorders