Fine Structure of a Genetic Region in Bacteriophage
Year: 1955
Type: article
Abstract: This paper describes a functionally related region in the genetic material of a bacteriophage that is finely subdivisible by mutation and by genetic recombination. The group of mutants resembles similar cases which have been observed in many organisms, usually designated as "pseudo-alleles." (See reviews by Lewis [1] and Pontecorvo [2].) Such cases are of special interest for their bearing on the structure and function of genetic determinants. \n \nThe phenomenon of genetic recombination provides a powerful tool for separating mutations and discerning their positions along a chromosome. When it comes to very closely neighboring mutations, a difficulty arises, since the closer two mutations lie to one another, the smaller is the probability that recombination between them will occur. Therefore, failure to observe recombinant types among a finite number of progeny ordinarily does not justify the conclusion that the two mutations are inseparable but can only place an upper limit on the linkage distance between them. A high degree of resolution requires the examination of very many progeny. This can best be achieved if there is available a selective feature for the detection of small proportions of recombinants. Some preliminary results are here presented of a program designed to extend genetic studies to the molecular (nucleotide) level.
Author: Seymour Benzer
Institution: Purdue University West Lafayette
Language: English
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2074046561