Work

High-Resolution NMR Spectroscopy of a GPCR in Aligned Bicelles

Year: 2006

Type: article

Abstract: Solid-state NMR spectra with single-site resolution of CXCR1, a G protein-coupled receptor (GPCR), were obtained in magnetically aligned phospholipid bicelles. These results demonstrate that GPCRs in phospholipid bilayers are suitable samples for structure determination by solid-state NMR. The spectra also enable studies of drug-receptor interactions.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja0606632

Authors: Sang Ho Park, Stefan Prytulla, Anna A. De Angelis, Jonathan Miles Brown, Hans Kiefer, Stanley J. Opella

Institution: University of California San Diego

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering

Awards: Resource for NMR Molecular Imaging of Proteins, Molecular Imaging of G-Protein-Coupled Receptors for Drug Development, Membrane Protein Structures in Phospholipid Bilayers by Solid State NMR, Structure, Dynamics, and Functions of the Viroporins Vpu from HIV and p7 from HCV, Structural Biology of Membrane Transport Proteins