Work

Changes in Drosophila melanogaster midgut proteins in response to dietary Bowman–Birk inhibitor

Year: 2007

Type: article

Abstract: The midgut proteome of Drosophila melanogaster was compared in larvae fed dietary Bowman-Birk inhibitor (BBI) vs. larvae fed a control diet. By using two-dimensional gel electrophoresis, nine differentially expressed proteins were observed, which were associated with enzymes or transport functions such as sterol carrier protein X (SCPX), ubiquitin-conjugating enzyme, endopeptidase, receptor signalling protein kinase, ATP-dependent RNA helicase and alpha-tocopherol transport. Quantitative real-time PCR verified differential expression of transcripts coding for six of the proteins observed from the proteomic analysis. BBI evidently affects expression of proteins associated with protein degradation, transport and fatty acid catabolism. We then tested the hypothesis that SCPX was critical for the Drosophila third instars' response to BBI treatment. Inhibition of SCPX caused the third instars to become more susceptible to dietary BBI.

Source: Insect Molecular Biology

DOI: 10.1111/j.1365-2583.2007.00750.x

Authors: H.‐M. Li, V. Margam, W. M. Muir, L. L. Murdock, B. R. Pittendrigh

Institution: Purdue University West Lafayette

Language: English

Topic: Insect Resistance and Genetics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Purdue University, Hardwood Tree Improvement and Regeneration Center, Purdue University