Get started
Work

Protection Afforded by an HIV Vaccine Candidate in Macaques Depends on the Dose of SIVmac251at Challenge Exposure

Year: 2013
Type: article
Abstract: We used the simian immunodeficiency virus mac251 (SIV(mac251)) macaque model to study the effect of the dose of mucosal exposure on vaccine efficacy. We immunized macaques with a DNA prime followed by SIV gp120 protein immunization with ALVAC-SIV and gp120 in alum, and we challenged them with SIV(mac251) at either a single high dose or at two repeated low-dose exposures to a 10-fold-lower dose. Infection was neither prevented nor modified following a single high-dose challenge of the immunized macaques. However, two exposures to a 10-fold-lower dose resulted in protection from SIV(mac251) acquisition in 3 out of 12 macaques. The remaining animals that were infected had a modulated pathogenesis, significant downregulation of interferon responsive genes, and upregulation of genes involved in B- and T-cell responses. Thus, the choice of the experimental model greatly influences the vaccine efficacy of vaccines for human immunodeficiency virus (HIV).
DOI: 10.1128/jvi.02863-12
Authors: Monica Vaccari, Brandon F. Keele, Steven E. Bosinger, Melvin N. Doster, Zhong-Min Ma, Justin Pollara, Anna Hryniewicz, Guido Ferrari, Yongjun Guan, Donald N. Forthal, David Venzon, Claudio Fenizia, Tia Morgan, David Montefiori, Jeffrey D. Lifson, Chris J. Miller, Guido Silvestri, Margherita Rosati, Barbara K. Felber, George N. Pavlakis, James Tartaglia, Genoveffa Franchini
Institutions: National Institutes of Health, National Cancer Institute, Frederick National Laboratory for Cancer Research, Emory University, Emory National Primate Research Center, University of California, Davis, Duke University, University of Maryland, Baltimore, University of California, Irvine
Language: English
Subfield: Virology
Open Access status: green
OpenAlex ID: W2075075085
FWCI: 4.88
Cites: 48
Cited by: 53
Related to: 10