Work

Poly(ADP-ribose) polymerase-1 dependence of stress-induced transcription factors and associated gene expression in glia

Year: 2002

Type: article

Abstract: Poly(ADP-ribose) polymerase-1 (PARP-1, EC ), a nuclear enzyme activated by DNA strand breaks, physiologically participates in DNA repair. Excessive activation of PARP-1 by cellular insults depletes its substrate beta-nicotinamide adenine dinucleotide and ATP, leading to cell death. PARP-1-deficient (PARP-1-/-) mice are protected from several forms of inflammation. In the present study, we demonstrate in PARP-1-/- glial cells a loss of several stress-activated transcription factors as well as decreased expression of genes for cytokines and cellular adhesion molecules. We also show that augmented expression of some of these genes is independent of PARP-1 catalytic activity. These findings indicate that PARP-1 plays a pivotal role in the initial inflammatory response by modulating transcription of inflammation-linked genes.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.052712399

Authors: Hyo Chol Ha, Lynda D. Hester, Solomon H. Snyder

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: PARP inhibition in cancer therapy

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Johns Hopkins University, U.S. Public Health Service, National Institute on Drug Abuse