Year: 2009
Type: conference-paper
Abstract: The Scanning electrochemical microscopy (SECM) is used to selectively detect intervening mismatches and abasic sites within monolayers of ds-DNA self-assembled onto gold electrodes. The electrocatalytic cycle involving oxidation of leucomethylene blue (LB), an electrochemically reduced form of methylene blue intercalated within the ds-DNA monolayer self-assembled on gold electrode, by the SECM tip-generated ferricyanide allows regeneration of LB via long-range heterogenous electron transfer mediated by the DNA π-stack. Monolayers featuring disruptions in the DNA π-stack via a single-base mismatch or abasic site exhibit strongly attenuated methylene blue electrochemistry within the DNA film leading to a disruption of the catalytic cycle, thereby diminishing the SECM tip current. The inherent differences in catalytic tip currents can be used to distinguish monolayer domains containing native ds-DNA versus ds-DNA containing π-stack lesions within the micron-level lateral spatial resolution of SECM.
Source: ECS Transactions
DOI: 10.1149/1.3103810
Authors: William Hammond, Justin Arndt, Thi Nguyen, Katarzyna Slowinska, Christopher Jackson, Hayden Burgoyne, Michael Hill, Krzysztof Slowinski
Institutions: California State University, Long Beach, Occidental College
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2075463343