Work

Effect of sodium and calcium chlorides, abscisic acid and proline on callus cultures ofArachis hypogaea L.

Year: 1996

Type: article

Abstract: Callus cultures ofArachis hypogaea L. cv. JL-24 adapted to 200 mM NaCl (otherwise lethal to cells) were used for the study. Calli grew slowly when transferred to 250 mM NaCl, but the growth was enhanced when ABA was included in the medium. ABA induced increase in growth of callus was not accompanied by corresponding increase in internal free proline levels. 0.5 mM of CaCl2 ameliorated the negative effect of NaCl indicating that cells require a specific Ca2+/Na+ ratio for their growth. Proline content also increased at this ratio thereby suggesting that increase in growth at 0.5 mM Ca2+ may be due to an increase in proline content. However, exogenous proline did not increase the growth of callus (adapted to 200 mM), and higher concentrations even inhibited the growth. This shows that proline is not required for growth or adaptation of cells to salt stress, but is produced as a consequence of stress.

Source: Biologia Plantarum

DOI: 10.1007/bf02890601

Authors: A. Gupta, G. S. Singhal

Institution: Jawaharlal Nehru University

Language: English

Topic: Plant Stress Responses and Tolerance

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funder: Council of Scientific and Industrial Research, India