Work

The Abnormal Spindle-like, Microcephaly-associated (ASPM) Gene Encodes a Centrosomal Protein

Year: 2005

Type: article

Abstract: ASPMabnormal spindle-like, microcephaly associated asp abnormal spindles CENPJ centromere-associated protein J CPAP centrosomal protein 4.1-associated protein MCPH primary microcephaly PTCB proteins with twin carboxylterminal BRCT domains siRNA small interfering RNA CDK5RAP2 cyclin dependent kinase 5 regulatory associated protein Brief ReportThe Abnormal Spindle-Like, Microcephaly-Associated (ASPM) Gene Encodes a Centrosomal Protein ABSTRACTHomozygous mutations in the abnormal spindle-like, microcephaly-associated ASPM gene are the leading cause of autosomal recessive primary microcephaly.ASPM is the putative human ortholog of the Drosophila melanogaster abnormal spindles gene (asp), which is essential for mitotic spindle function.Here, we report that downregulation of endogenous ASPM by siRNA decreases protein levels of endogenous BRCA1.ASPM localizes to the centrosome in interphase and to the spindle poles from prophase through telophase.These findings indicate that ASPM may be involved in mitotic spindle function, possibly, through regulation of BRCA1.

Source: Cell Cycle

DOI: 10.4161/cc.4.9.2029

Authors: Xueyan Zhong, Limin Liu, Ailian Zhao, Gerd P. Pfeifer, Xingzhi Xu

Institutions: City Of Hope National Medical Center, Beckman Research Institute, Peking University Cancer Hospital

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, National Cancer Institute