Work

Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes.

Year: 1988

Type: article

Abstract: Using morphological and cell biological techniques, we have shown that the RNA replicase of Semliki Forest and Sindbis virus (two closely related alphaviruses) is located in complex ribonucleoprotein structures associated with the cytoplasmic surface of modified secondary lysosomes and endosomes. These nucleoprotein complexes often form a bridge between the membrane of the endocytic vacuole and the rough endoplasmic reticulum where the synthesis of the structural proteins of these viruses occurs. The results suggest that these cytopathic vacuoles constitute sites not only for viral RNA synthesis, but also for translation of structural proteins, and for the assembly of nucleocapsids.

Source: The Journal of Cell Biology

DOI: 10.1083/jcb.107.6.2075

Authors: S Froshauer, J Kartenbeck, A Helenius

Institution: Yale University

Language: English

Topic: Mosquito-borne diseases and control

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: PROTEIN FOLDING IN THE ENDOPLASMIC RETICULUM, G3330774117