Work

The Histone Gene Transcription Factor HiNF-P Stabilizes Its Cell Cycle Regulatory Co-Activator p220NPAT

Year: 2006

Type: article

Abstract: Orderly progression through the cell cycle requires the transcriptional activation of histone genes to support packaging of newly replicated DNA. Induction of human histone gene expression is mediated by a co-activation complex containing transcription factor HiNF-P and its cofactor p220NPAT. Here, using cells synchronized in S-phase and in mitosis, as well as serum-stimulated cells, we have investigated how HiNF-P is regulated during the cell cycle and examined its stability relative to p220NPAT. We find that while HiNF-P is maintained at steady-state levels throughout the cell cycle, both HiNF-P and p220NPAT are actively degraded by the proteasome pathway. Importantly, elevation of HiNF-P levels enhances the stability of its co-activator p220NPAT. The HiNF-P-dependent stabilization of p220NPAT may reinforce signaling through the cyclin E/CDK2/p220NPAT pathway and contribute to coordinate control of histone gene expression.

Source: Biochemistry

DOI: 10.1021/bi061425m

Authors: Ricardo Medina, Andre J. van Wijnen, Gary S. Stein, Janet L. Stein

Institution: University of Massachusetts Chan Medical School

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: University of Massachusetts Medical School, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: G2677314950, Identification of Histone Gene Regulatory Sequences, Diabetes Endocrinology Research Center (DERC), G5995022133