Impact of Dissociation Constant on the Detection Sensitivity of Polymerization-Based Signal Amplification Reactions
Year: 2013
Type: article
Abstract: Many studies have demonstrated the concept of using free-radical polymerization reactions to provide signal amplification so that molecular recognition events indicative of disease states may be detected in a simple and low-cost manner. We provide the first systematic study of how the dissociation constant impacts detection sensitivity in these assays, having chosen a range of dissociation constants (nanomolar to picomolar) that is typical of those encountered in molecular diagnostic applications that detect protein-protein binding events. In addition, we use experimental results to validate a mass-action kinetic model that may be used to predict assay performance as an alternative or supplement to the empirical approach to developing new polymerization-based amplification assays that has characterized the field to date.
Source: Analytical Chemistry
DOI: 10.1021/ac4018988
Authors: Kaja Kaastrup, Leslie Chan, Hadley D. Sikes
Institution: Massachusetts Institute of Technology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2077225118