Work

Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing

Year: 1999

Type: article

Abstract: PCR-ribotying, a typing method based on polymorphism in the 16S-23S intergenic spacer region, has been recently used to investigate outbreaks due to Clostridium difficile. However, this method generates bands of high and close molecular masses which are difficult to separate on agarose gel electrophoresis. To improve reading of banding patterns of PCR-ribotyping applied to C. difficile, a partial sequencing of the rRNA genes (16S and 23S) and intergenic spacer region has been performed, then a new set of primers located closer to the intergenic spacer region has been defined. The new PCR gave reproducible patterns of bands easy to separate on agarose gel electrophoresis. Each of the 10 serogroups and 11 subgroups of serogroup A produced a different pattern. This typing method has evidenced major qualities such as easiness, rapidity and reproducibility. However, its discriminatory power has to be evaluated to validate its importance as a typing tool for C. difficile.

Source: FEMS Microbiology Letters

DOI: 10.1016/s0378-1097(99)00204-9

Authors: Philippe Bidet, FrÃ©dÃ©ric Barbut, ValÃ©rie Lalande, BÃ©atrice Burghoffer, Jean-Claude Petit

Institutions: Assistance Publique – Hôpitaux de Paris, Hôpital Saint-Antoine, Université Pierre-et-Marie-Curie

Language: English

Topic: Clostridium difficile and Clostridium perfringens research

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2077265045