Work

Direct Acylation of Carrier Proteins with Functionalized β-Lactones

Year: 2010

Type: article

Abstract: As the key component of many biosynthetic assemblies, acyl-carrier proteins offer a robust entry point for introduction of small molecule probes and pathway intermediates. Current labeling strategies primarily rely on modifications to the phosphopantetheine cofactor or its biosynthetic precursors followed by attachment to the apo form of a given carrier protein. As a greatly simplified alternative, direct and selective acylation of holo-acyl-carrier proteins using readily accessible beta-lactones as electrophilic partners for the phosphopantetheine-thiol has been demonstrated.

Source: Organic Letters

DOI: 10.1021/ol100684s

Authors: Jon W. Amoroso, Lawrence S. Borketey, Gitanjeli Prasad, Nathan A. Schnarr

Institution: University of Massachusetts Amherst

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: Chemistry Biology Interface Predoctoral Training Grant