Ultrastructural visualization of virus-induced surface antigens. Comparative studies of three immunohistochemical techniques.
Year: 1975
Type: article
Abstract: Three immunohistochemical techniques used for ultrastructural localization of cell surface antigens were compared with respect to ease of reagent preparation and qualitative aspects of cell surface staining. The techniques compared were: (a) ferritin-conjugated antibody, (b) "hybrid" antibody and (c) a modification of the "bridging" technique which employs soluble immune complexes. Used in conjuction with strain MC29 avian oncornavirus-infected chick embryo cells and chicken antiviral immune serum, the results of all three techniques were comparable. In terms of ease of preparation, the "bridging" technique employing soluble complexes was found to be the most satisfactory.
DOI: 10.1177/23.11.172555
Authors: R E Morris, R B Fritz
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: bronze
Funder: National Cancer Institute
OpenAlex ID: W2077829374