Work

Two postprocessing techniques for the elimination of background autofluorescence for fluorescence lifetime imaging microscopy

Year: 2008

Type: article

Abstract: The analysis of fluorescence lifetime imaging microscopy (FLIM) data under complex biological conditions can be challenging. Particularly, the presence of short-lived autofluorescent aggregates can confound lifetime measurements in fluorescence energy transfer (FRET) experiments, where it can become confused with the signal from exogenous fluorophores. Here we report two techniques that can be used to discriminate the contribution of autofluorescence from exogenous fluorphores in FLIM. We apply the techniques to transgenic mice that natively express yellow fluorescence protein (YFP) in a subset of cortical neurons and to histological slices of aged human brain tissue, where we study the misfolding of intracellular tau protein in the form of neurofibrillary tangles.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.2837169

Authors: Phill B. Jones, Aneta Rozkalne, Melanie Meyer-Luehmann, Tara L. Spires-Jones, Alexandra Makarova, Anand T. Kumar, Oksana Berezovska, Brian J. Bacskai, Bradley T. Hyman

Institutions: Harvard University, Massachusetts General Hospital, Athinoula A. Martinos Center for Biomedical Imaging

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Dana Foundation, University of Pennsylvania, National Institutes of Health, U.S. Public Health Service, National Institute on Aging, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering

Awards: G1412165948, Anatomical Changes in Tau Transgenic Models, G1915837458, G2132047791, G279242282, Multiphoton microscopy for in vivo neural imaging, G39712242, G4413556686, G6703509384, Non-invasive optical imaging of neuropathology in vivo, G7237067214, Molecular mechanism of Presenilin-1 (PS1) linked Alzheimer's Disease pathology, G9000140468, G9000220226, G9000313697, G942089662

OpenAlex ID: W2078437878

FWCI: 0.865

Cites: 30

Cited by: 17

Related to: 10