Work

A Complex of Methylthioadenosine/S-Adenosylhomocysteine Nucleosidase, Transition State Analogue, and Nucleophilic Water Identified by Mass Spectrometry

Year: 2012

Type: article

Abstract: An enzyme-stabilized nucleophilic water molecule has been implicated at the transition state of Escherichia coli methylthioadenosine nucleosidase (EcMTAN) by transition state analysis and crystallography. We analyzed the EcMTAN mass in complex with a femtomolar transition state analogue to determine whether the inhibitor and nucleophilic water could be detected in the gas phase. EcMTAN-inhibitor and EcMTAN-inhibitor-nucleophilic water complexes were identified by high-resolution mass spectrometry under nondenaturing conditions. The enzyme-inhibitor-water complex is sufficiently stable to exist in the gas phase.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja211176q

Authors: Shanzhi Wang, Jihyeon Lim, Keisha Thomas, Funing Yan, Ruth H. Angeletti, Vern L. Schramm

Institution: Albert Einstein College of Medicine

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Center for Research Resources