Work

Identification of the NH2‐terminal blocking group of calcineurin B as myristic acid

Year: 1982

Type: article

Abstract: The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-Gly-Asn-Glu-Ala-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

Source: FEBS Letters

DOI: 10.1016/0014-5793(82)80759-x

Authors: Alastair Aitken, Philip Cohen, Sitthivet Santikarn, Dudley H. Williams, A.Graham Calder, Alistair Smith, Claude B. Klee

Institutions: University of Dundee, University of Cambridge, National Institutes of Health, National Cancer Institute

Language: English

Topic: Signaling Pathways in Disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Medical Research Council

OpenAlex ID: W2079159599

FWCI: 5.82

Cites: 9

Cited by: 303

Related to: 9