Work

Metabolic and signaling properties of an Itpk gene family in Glycine max

Year: 2008

Type: article

Abstract: We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to interconvert the Cl(-) channel inhibitor, Ins(3,4,5,6)P4, and its metabolic precursor, Ins(1,3,4,5,6)P5, within a substrate cycle. The soybean Itpks also phosphorylated Ins(3,4,6)P3 to Ins(1,3,4,6)P4 which was further phosphorylated to Ins(1,3,4,5,6)P5 by soybean Ipk2. Thus, soybean Itpks may participate in an inositol lipid-independent pathway of InsP6 synthesis.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2008.04.054

Authors: Amanda R. Stiles, Xun Qian, Stephen B. Shears, Elizabeth A. Grabau

Institutions: Virginia Tech, National Institutes of Health, National Institute of Environmental Health Sciences

Language: English

Topic: Plant nutrient uptake and metabolism

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: bronze

Funders: U.S. Department of Agriculture, United Soybean Board, National Institutes of Health, National Institute of Environmental Health Sciences

Award: Inositol Signalling