Dynamic profiling of the protein life cycle in response to pathogens
Year: 2015
Type: article
Abstract: Protein expression is regulated by the production and degradation of messenger RNAs (mRNAs) and proteins, but their specific relationships remain unknown. We combine measurements of protein production and degradation and mRNA dynamics so as to build a quantitative genomic model of the differential regulation of gene expression in lipopolysaccharide-stimulated mouse dendritic cells. Changes in mRNA abundance play a dominant role in determining most dynamic fold changes in protein levels. Conversely, the preexisting proteome of proteins performing basic cellular functions is remodeled primarily through changes in protein production or degradation, accounting for more than half of the absolute change in protein molecules in the cell. Thus, the proteome is regulated by transcriptional induction for newly activated cellular functions and by protein life-cycle changes for remodeling of preexisting functions.
Source: Science
Authors: Marko Jovanovic, Michael S. Rooney, Philipp Mertins, Dariusz Przybylski, Nicolas Chevrier, Rahul Satija, Edwin H. Rodriguez, Alexander P. Fields, Schraga Schwartz, Raktima Raychowdhury, Maxwell R. Mumbach, Thomas Eisenhaure, Michal Rabani, Dave Gennert, Diana Lu, Toni Delorey, Jonathan S. Weissman, Steven A. Carr, Nir Hacohen, Aviv Regev
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Funders: Howard Hughes Medical Institute, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, National Institutes of Health, Klarman Cell Observatory, Broad Institute, National Human Genome Research Institute, National Institute of General Medical Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development
OpenAlex ID: W2079279399