Work

Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.

Year: 1978

Type: article

Abstract: HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equal to that of serum-supplemented medium. The omission of any one of the five components results in less than maximal cell growth.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.75.2.901

Authors: S E Hutchings, G H Sato

Language: English

Topic: Viral Infectious Diseases and Gene Expression in Insects

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2080118074

FWCI: 8.76

Cites: 16

Cited by: 211

Related to: 10