Work

Molecular Etching: A Novel Methodology for the Generation of Complex Micropatterned Growth Surfaces for Human Cellular Assays

Year: 2014

Type: article

Abstract: The extracellular matrix (ECM) is the noncellular component of all tissues. In vitro, micropatterning of ECM has enabled the control of cell shape and function. Here, a novel methodology to reliably micropattern complex, biologically active ECM protein mixtures on a variety of growth substrates is presented. It is further shown that these conditions provide more physiologic cues than single purified proteins.

Source: Advanced Healthcare Materials

DOI: 10.1002/adhm.201400010

Authors: Ayhan Atmanli, Dongjian Hu, Ibrahim J. Domian

Institutions: Harvard University, Massachusetts General Hospital, Harvard Stem Cell Institute

Language: English

Topic: Cellular Mechanics and Interactions

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Heart, Lung, and Blood Institute

Awards: Human Pluripotent Stem Cell and Progenitor Models of Cardiac and Blood Diseases, Control of Cardiac Lineage Commitment and Differentiation in Murine Development