Work

Cdc42 interacting protein 4 (CIP4) is essential for integrin-dependent T-cell trafficking

Year: 2010

Type: article

Abstract: The F-BAR domain containing protein CIP4 (Cdc42 interacting protein 4) interacts with Cdc42 and WASP/N-WASP and is thought to participate in the assembly of filamentous actin. CIP4(-/-) mice had normal T- and B-lymphocyte development but impaired T cell-dependent antibody production, IgG antibody affinity maturation, and germinal center (GC) formation, despite an intact CD40L-CD40 axis. CIP4(-/-) mice also had impaired contact hypersensitivity (CHS) to haptens, and their T cells failed to adoptively transfer CHS. Ovalbumin-activated CD4(+) effector T cells from CIP4(-/-)/OT-II mice migrated poorly to antigen-challenged skin. Activated CIP4(-/-) T cells exhibited impaired adhesion and polarization on immobilized VCAM-1 and ICAM-1 and defective arrest and transmigration across murine endothelial cell monolayers under shear flow conditions. These results demonstrate an important role for CIP4 in integrin-dependent T cell-dependent antibody responses and GC formation and in integrin-mediated recruitment of effector T cells to cutaneous sites of antigen-driven immune reactions.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1002747107

Authors: Suresh Koduru, Lalit Kumar, Michel J. Massaad, Narayanaswamy Ramesh, Séverine Le Bras, Esra Ozcan, Michiko K. Oyoshi, Mayumi Kaku, Yuko Fujiwara, Leonor Kremer, Sandra King, Robert Fuhlbrigge, Scott Rodig, Peter Sage, Chris Carman, Pilar Alcaide, Francis W. Luscinskas, Raif S. Geha

Institutions: Boston Children's Hospital, Harvard University, Brigham and Women's Hospital, Consejo Superior de Investigaciones Científicas, Centro Nacional de Biotecnología, Beth Israel Deaconess Medical Center, Center for Vascular Biology Research

Language: English

Topic: Cell Adhesion Molecules Research

Subfield: Immunology and Allergy

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: American Heart Association, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Arthritis and Musculoskeletal and Skin Diseases

Awards: G2000825783, G2580345468, G2784508480, SIGNALING AND CYTOSKELETAL ORGANIZATION IN BLOOD CELLS, LRRC8 Signaling in T Cells, Downstream of Akt in the tumor vessel, Targeted siRNA delivery to aberrantly activated leukocytes for treating colitis, G4425807348, Skin Homing T Cells -- Molecular Characterization of CLA, G6294206292, G7327152327, G734330516, Analysis of Class II MHC and CD1d antigen presentation pathways in skin-derived d, Harvard Longwood Medical Area Training Grant in Transplantation

OpenAlex ID: W2080317597

FWCI: 1.935

Cites: 30

Cited by: 27

Related to: 10