Work

Computational design of orthogonal nucleoside kinases

Year: 2010

Type: article

Abstract: We report the computational enzyme design of an orthogonal nucleoside analog kinase for 3'-deoxythymidine. The best kinase variant shows an 8500-fold change in substrate specificity, resulting from a 4.6-fold gain in catalytic efficiency for the nucleoside analog and a 2000-fold decline for the native substrate thymidine.

Source: Chemical Communications

DOI: 10.1039/c0cc02961k

Authors: Lingfeng Liu, Paul Murphy, David Baker, Stefan Lutz

Institutions: Emory University, University of Washington, Howard Hughes Medical Institute

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institute of General Medical Sciences

Awards: G4488912935, Improving nucleoside phosphorylation with hybrid kinases