Work

2H-DNP-enhanced 2H–13C solid-state NMR correlation spectroscopy

Year: 2010

Type: article

Abstract: Perdeuteration of biological macromolecules for magic angle spinning solid-state NMR spectroscopy can yield high-resolution (2)H-(13)C correlation spectra and the method is therefore of great interest for the structural biology community. Here we demonstrate that the combination of sample deuteration and dynamic nuclear polarization yields resolved (2)H-(13)C correlation spectra with a signal enhancement of epsilon > or = 700 compared to a spectrum recorded with microwaves off and otherwise identical conditions. To our knowledge, this is the first time that (2)H-DNP has been employed to enhance MAS-NMR spectra of a biologically relevant system. The DNP process is studied using several polarizing agents and the technique is applied to obtain (2)H-(13)C correlation spectra of U-[(2)H, (13)C] proline.

Source: Physical Chemistry Chemical Physics

DOI: 10.1039/c003705b

Authors: Thorsten Maly, Loren B. Andreas, Albert A. Smith, Robert G. Griffin

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering

Awards: Solid State NMR Studies of Peptides and Proteins, G1721210722, NMR Microscopy of Single Neural Cells and Brain Slices, MIT/ Harvard Center for Magnetic Resonance, High Field DNP and EPR in Biological Systems, G549907944, Solid State NMR Studies of Membrane Proteins

OpenAlex ID: W2080709387

FWCI: 3.817

Cites: 60

Cited by: 61

Related to: 10