Optimization of Antioxidant Silk Fibroin Peptide Hydrolysis Process
Year: 2014
Type: article
Abstract: Papain, pancreatin, pepsin, rypsin, neutral protease were used for the enzymatic hydrolysis of silk fibroin solution. It was found that the silk fibroin peptides after hydrolysis by pancreatin exhibited the strongest antioxidant activity, in comparison of five hydrolysed solutions by the pyrogallol method. The pancreatin hydrolysis process was then optimised. The optimum conditions of pancreatin hydrolysis antioxidant activity silk fibroin peptide was determined by orthogonal experiment: reaction time 160min, enzyme concentration 4%,substrate concentration 20mg/ml, pH 8, temperature 38°C. The best hydrolysis under the hydrolyzate on superoxide radical scavenging rate of 72.73%.
Source: Advanced materials research
Authors: Zhen Zhu, Da Peng Wang, Yuan Ma, Xu Yang, Xiao Juan Wang, Hua Yin, Yan An
Institutions: Soochow University, Soochow University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2080776236