Year: 2010
Type: article
Abstract: Background: Neurocysticercosis is the infection caused by the larval stage of Taenia solium and is also the most common parasite of the nervous system in humans and is a public health problem in poor areas of Latina America, Asia and Africa and it is spreading worldwide due to increased migration of NC cases and tapeworm carriers. T. solium eggs contain an oncosphere that is released and penetrates in the host small intestine. This mechanism is not known. Therefore, the elucidation of the molecules involved in the parasite-host cell recognition process is of extreme importance and that constitute a focal point of research. Previous works supported the idea that parasites can interact with extracellular matrix and basement membrane proteins, such as fibronectin (FN), laminin, collagen and vitronectin leading to enhanced adherence. The purpose of this study was evaluated the role of FN in the adherence mechanism of T. solium oncosphere using in vitro assay in CHO-K1 cell. Methods: The T. solium oncospheres and CHO-K1 cell monolayer were incubated with different concentrations of FN (70, 80, 90 and 100 ug/ml) and the adherence was visualized by immunofluorescence using hyperimmune rabbit sera against T. solium activated oncospheres. In the other hand, only the CHO –K1 cells were preincubated with 80 ug/ml of FN for 20 h and then the T. solium oncospheres with and without FN were added on monolayer cell. FN fibrils and oncospheres were visualized by immunofluorescence using polyclonal anti-FN antibody produced in rabbit by UV light microscopy. Results: The adherence of T. solium oncospheres was increased from 70 ug/ml FN versus control. The fibrillar FN matrix on CHO–K1 cells were visualized over monolayer cell. Also, the adherence T. solium oncosphere with FN preincubate reacted with anti-FN antibody but with minor intensity versus control. Conclusion: These results suggest that Fn increases the adherence of oncosphere of T. solium serving as a bridge between the T. solium oncosphere and cell. Additonal characterization of T. solium oncosphere fibronectin binding protein that interact with host components, will further our understanding of the adherence and could be markers as candidate a vaccines. Abstracts for SupplementInternational Journal of Infectious DiseasesVol. 14Preview Full-Text PDF Open Archive
Language: English
Subfield: Pathology and Forensic Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,900
OpenAlex ID: W2081014249