Work

Integration of Rous Sarcoma Virus DNA: a CA Dinucleotide Is Not Required for Integration of the U3 End of Viral DNA

Year: 2008

Type: article

Abstract: The two ends of RSV linear DNA are independently inserted into host DNA by integrase in vivo. We previously showed that the range of U3 sequences that are acceptable substrates for integrase appeared to be greater than the range of acceptable U5 sequences in vivo. We have done additional experiments to determine which U3 sequences are good integrase substrates. On the U3 end, there does not appear to be a stringent requirement for the canonical CA, integrase can efficiently remove three nucleotides, and six nucleotides are sufficient to allow integration with reasonable, albeit reduced, efficiency.

Source: Journal of Virology

DOI: 10.1128/jvi.01353-08

Authors: Jangsuk Oh, Kevin W. Chang, Stephen H. Hughes

Institutions: National Heart, Lung, and Blood Institute, National Cancer Institute, National Institutes of Health, Office of Technology Transfer

Language: English

Topic: HIV Research and Treatment

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

OpenAlex ID: W2081027720

FWCI: 0.225

Cites: 24

Cited by: 7

Related to: 10